Smo promotes the translocation of OFD1 from satellites to the centrioles via Gα_i-LGN-NuMA-dynein protein complex. (A) SIM images (top view) indicating that OFD1 localizes at centriolar satellites using the satellite marker PCM1, and its translocation to distal end of centrioles upon Hh activation using Ninein as subdistal appendage marker. The yellow dashed lines indicate the centriolar satellite area. The cartoon figures indicate how OFD1 looks from different views with respect to other centriolar proteins. (B) SIM images indicating OFD1 localization with respect to γ Tubulin as centriole marker from top view in RPE1 WT, Smo KO, and cells depleted of LKB1, AMPK, Gα_{i (1+2+3)}, LGN, and NuMA and treated with Ciliobrevin A (100 nM) at LCD, with or without Shh after 5 h. The cartoon figures are to facilitate better understanding of OFD1 changes in each condition. The red boxes indicate the centriolar satellite area. Yellow and white arrows indicate the presence or absence of OFD1 at centrioles, respectively.