OFD1 localization at satellites and centrioles is redistributed upon Hh activation. (A) Confocal stitched images of RPE1 cells to provide a bigger field of view, indicating OFD1 distribution in cells at different conditions as indicated. Cells were plated at LCD, treated with or without Shh for 7 h, and stained for OFD1 and γ Tubulin. OFD1 accumulation decreases at satellites and increases at centrioles upon Hh activation. (B) SIM images indicating OFD1 localization in respect to γ Tubulin as centriole marker in RPE1 WT treated with or without Shh for 5 h; top view from different angles. (C) SIM images indicating OFD1 localization in RPE1 cells at LCD and treated with or without Shh after 24 h when cilium was formed. (D) SIM images indicating BBS4 localization in respect to γ Tubulin as centriole marker in RPE1 WT or Gα_i-depleted cells treated with Shh for 5 h; top view from different angles. Unlike WT cells, BBS4 does not localize at centrioles, despite proximity with centrioles.