Smo activation of autophagy via LKB1 promotes the degradation of OFD1. (A) Western blots probed for OFD1 or LC3 to assess degradation of OFD1 or activation of autophagy, respectively, at different conditions as indicated. Cell were treated with or without Shh for different time periods as indicated. Black lines separate nonconsecutive parts of the same sets of Western blots. (B) Quantifications of OFD1 intensity for each Western blot shown in A as indicated. (C) Confocal images (stitched) of RPE1 cells indicating OFD1 distribution in cells plated at LCD and treated with or without Shh for 5 h, stained for OFD1 and γ Tubulin. OFD1 is removed from satellites, while its accumulation increases at centrioles upon Hh activation. Gα_i siRNA reparents KD of different isoforms, Gα_{i (1+2+3)}. (D) Quantification of OFD1 total cell intensity, and OFD1 centriole intensity at different conditions as indicated. The quantifications were done using images taken with confocal microscopy. *, P < 0.01; **, P < 0.001, comparing Shh treated cells in each condition to those without Shh in the same condition . Number of cells (n) used for each experiment (three to four repeats) is listed in Table S4.