The CSD shows a distinct lipid specificity, colocalizes with CAV1, and affects PM lipid organization. (A) Bivariate analysis: CSD coclusters with lipids and CAV1 (the left panel indicates raw data and the right panel shows the bivariate coclustering summary). (B–F) CSD affects PtdIns(4,5)P₂, PtdIns(3,4,5)P₃, and cholesterol nanoclustering. (G) Lipid univariate clustering summary. (H) CSD affects surface levels of PS and cholesterol. Data summaries in A, G, and H show mean ± SEM. Bootstrap tests examined the statistical differences between conditions in the spatial distribution data in A and G, with * indicating P < 0.05. For gold density in H, one-way ANOVA was used to evaluate the statistical differences, with * indicating P < 0.05.