Ribosome reconstruction and polysome orientation. (A) Reconstructed ribosome (light blue) fitted into the 80S ribosome volume from EMD-3420 (orange mesh). (B) Reconstructed ribosome volume with depicted 40S (light blue) and 60S (dark blue) subunits and L7/L12 stalk. (C) Rotated view of ribosome from B. Putative path of mRNA depicted in green; white arrow shows L7/L12 stalk. (D) Polysomes found in various tomograms. (E) Estimation of reconstructed ribosome resolution, FSC curve. (F and G) Slices from tomogram of ribosomes on the surface of ER. (H) Segmented view of F and G. Scale bars: 10 nm (A–C); 25 nm (D); 100 nm (F–H).
Figure 5.

Ribosome reconstruction and polysome orientation. (A) Reconstructed ribosome (light blue) fitted into the 80S ribosome volume from EMD-3420 (orange mesh). (B) Reconstructed ribosome volume with depicted 40S (light blue) and 60S (dark blue) subunits and L7/L12 stalk. (C) Rotated view of ribosome from B. Putative path of mRNA depicted in green; white arrow shows L7/L12 stalk. (D) Polysomes found in various tomograms. (E) Estimation of reconstructed ribosome resolution, FSC curve. (F and G) Slices from tomogram of ribosomes on the surface of ER. (H) Segmented view of F and G. Scale bars: 10 nm (A–C); 25 nm (D); 100 nm (F–H).

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