Fluorescence intensities of WT and cK0 GPCR variants at t = 0. (A) Ciliary ^APSSTR3 was pulse labeled by addition of Alexa Fluor 647–conjugated mSA (mSA647) to the medium for 5–10 min before addition of sst. Violin plots of the ciliary fluorescence intensities for WT and cK0 SSTR3 imaged in the far-red channel at t = 0 are shown. n = 18–22 cilia. (B) Violin plots of the fluorescence intensities of NG fluorescence corresponding to WT and cK0 GPR161^NG at t = 0 are shown. n = 10–19 cilia. RFU, relative fluorescence unit.