17 Drosophila centrosome proteins were selected for binary complex screening. (A) Cartoon shows the 17 centrosome proteins that were selected for binary complex solubility screening and their positions within the centrosome. (B) Schematic of the proteins tested in the screen. Numbers show amino acids, blue regions predict coiled-coils, additional structural/functional domains are indicated, and horizontal lines show the locations where proteins were subdivided into smaller testable fragments (F1–5). Also described in Galletta et al. (2016). (C) PCR products used for random plasmid assembly. Each gene was amplified as FL and/or sub-fragments using primers listed in Table S2. L1F and L2R primers were used to generate 53 unique PCR products that could occupy the “gene A” position. L2F and L3R primers were used to generate 53 unique PCR products that could occupy the “gene B” position. pExpress A and B PCR fragments were used to assemble the plasmid backbone. (C′) Schematic of the final plasmid indicating the positions of the His₆-geneA and geneB-GFP, primers, and the ribosome binding sites (RBS1 and RBS2).