NEURL4-mediated regulation of mitochondrial proteins by PARylation. (A and B) SILAC-based enrichment of PARylated proteins in the absence of exogenous genotoxic stress. Most significant gene ontology (GO) terms associated with proteins with decreased PARylation in the absence of NEURL4 (A). Most significant pathways associated with decreased PARylation in the absence of NEURL4 (B). GO terms and KEGG pathways enrichment are based on a combined score between P value and Z score (DAVID). (C) Immunostaining showing mitochondrial network changes in HeLa cells in the absence of NEURL4. (D) Decreased basal respiration and impaired FCCP response in HeLa N4-KO cells by Seahorse. (E) Most significant gene sets from the Molecular Signature Database (MSigDB) enriched among DEGs between WT and N4-KO HeLa cells. CC, cellular components; FA, fatty acid; OCR, oxygen consumption rate; EMT, epithelial-mesenchimal transition; KEGG, Kyoto Encyclopedia of Genes and Genomes; DAVID, Datavase for Annotation, Visualization, and Integrated Discovery.