FISH probes give a range of signal types/appearance in WT RPE cells. (A and B) Montage of chromosome 7 α-satellite (A) or 19q13.42 (B) FISH signals obtained from a single image field, with corresponding skeleton images and skeleton lengths. The images were arranged in ascending order of skeleton length. Note the change in shape, punctation, and length of FISH signals with the increase in the skeleton dot length scores. (C and D) shows the distribution of skeleton dot lengths obtained for chromosome 7 α-satellite and 19q13.42 FISH signals in WT RPE cells. Dashed lines indicate the cutoffs for defining the categories compact, diffused, and dispersed for chromosome 7 α-satellite and compact/diffused, dispersed, and scattered for 19q13.42 probe. Numbers of foci quantified for each sample (n) are as follows (in the order they appear on the bar graphs): C, n = 30; D, n = 30. Scale bar = 2 µm.
Figure 2.

FISH probes give a range of signal types/appearance in WT RPE cells. (A and B) Montage of chromosome 7 α-satellite (A) or 19q13.42 (B) FISH signals obtained from a single image field, with corresponding skeleton images and skeleton lengths. The images were arranged in ascending order of skeleton length. Note the change in shape, punctation, and length of FISH signals with the increase in the skeleton dot length scores. (C and D) shows the distribution of skeleton dot lengths obtained for chromosome 7 α-satellite and 19q13.42 FISH signals in WT RPE cells. Dashed lines indicate the cutoffs for defining the categories compact, diffused, and dispersed for chromosome 7 α-satellite and compact/diffused, dispersed, and scattered for 19q13.42 probe. Numbers of foci quantified for each sample (n) are as follows (in the order they appear on the bar graphs): C, n = 30; D, n = 30. Scale bar = 2 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal