Glycosylation of cysteine-rich proteins. (A and B) Distribution of glycosylation sites in cysteine (cys)–rich domains of cysteine-rich proteins. Cysteine-rich domains are defined in Materials and methods. (C) The Δlog₂ values for quantified sites in LRP1 and MPRI. Color-coded boxes specify the dataset and Δlog₂ range. (D) Quantified acceptor sites in LRP1 are displayed as color-coded circles that flank the domain diagram showing cysteine-rich (LDLRA and EGF_3) and cysteine-deficient (LDLRB) domains. Red lines designate disulfides. Glycosylation sites marked by asterisks were quantified in a single LC-MS/MS experiment. (E) Quantified acceptor sites in MPRI are shown as color coded circles that flank the domain diagram showing the cysteine-rich CIMR repeats. TM spans (D and E) are indicated by black bars.