Expression and quantification of fluorescent probes in rods. (A) Amino acid sequences of PMP probes. (B) Transmission image of retinal slice preparation. (C) Confocal image in XY and XZ of a live rod expressing EGFP. Colors are a heat map: red is highest fluorescence, blue is lowest. Axial intensity values (lower panel) were taken along black lines. (B and C) Scale bars, 10 µm. (D) Structure, name, binding energy, and estimated membrane affinity of the lipid groups. The myristoylation motif results in heterogeneous acylation in retina, with four acyl varieties. In mammalians, the proportions are 55% C14:2, 20% C12:0, 16% C14:1, and 8% C14:0, as determined from mass spec of Tα (Kokame et al., 1992; Neubert et al., 1992; Johnson et al., 1994; Neubert and Hurley, 1998; Lobanova et al., 2007). Throughout, Myr refers to the myristoylation motif and acyl refers to lipids. Two K_ds are given for prenyls, with that for carboxymethylated appearing in parentheses. Binding energies and membrane affinity estimates were based on in vitro partitioning experiments (Peitzsch and McLaughlin, 1993; Silvius and l’Heureux, 1994).