Figure 6. RagAGDP determines FLCN recruitment to lysosomes. (A) Representative immunofluorescence images showing the localization of FLCN and Rags in NPRL3 KO cells transfected with WT RagA or RagAT21L + WT RagC under basal growth and starved conditions. Bars, 10 µm. Insets are 10.8 µm wide. (B) Quantification of FLCN lysosome localization (percentage of cells with five or more FLCN spots that are Rag positive; mean ± SEM; n = 3 experiments; 27–31 cells per condition; ANOVA with Bonferroni’s post hoc test).
Figure 6.

RagAGDP determines FLCN recruitment to lysosomes. (A) Representative immunofluorescence images showing the localization of FLCN and Rags in NPRL3 KO cells transfected with WT RagA or RagAT21L + WT RagC under basal growth and starved conditions. Bars, 10 µm. Insets are 10.8 µm wide. (B) Quantification of FLCN lysosome localization (percentage of cells with five or more FLCN spots that are Rag positive; mean ± SEM; n = 3 experiments; 27–31 cells per condition; ANOVA with Bonferroni’s post hoc test).

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