FLCN selectively interacts with inactive RagA. (A) Representative Western blots of GST pulldowns in WT and NPRL3 KO cells transfected with HA-GST-RagA + HA-RagC in basal and starved conditions. Because of their high abundance, HA-GST-RagA and HA-RagC in the pulldown condition were detected by Ponceau S staining. Molecular masses are given in kilodaltons. (B) Quantification of FLCN abundance in Rag pulldown (**, P < 0.01; ANOVA with Bonferroni’s post hoc test).