Figure 2. Reintroduction of RagA into RagA+B KO cells results in starvation-dependent recruitment of FLCN to lysosomes. (A) Western blot analysis of WT, RagA+B KO, and RagA+B KO HeLa cells expressing HA-RagA. Molecular masses are given in kilodaltons. (B) Representative immunofluorescence images showing HA-RagA localization to LAMP1-labeled lysosomes. Bars, 10 µm. Insets are 10.2 µm wide. (C) Representative immunofluorescence experiment showing the effect of starvation on FLCN lysosome localization in cells expressing HA-RagA. Bars, 10 µm. Insets are 10.2 µm wide. (D) Quantification of FLCN lysosome localization (percentage of cells with five or more FLCN spots that are also RagA positive; mean ± SEM; n = 3 experiments; 33–43 cells per condition; ***, P < 0.001; Student's t test).
Figure 2.

Reintroduction of RagA into RagA+B KO cells results in starvation-dependent recruitment of FLCN to lysosomes. (A) Western blot analysis of WT, RagA+B KO, and RagA+B KO HeLa cells expressing HA-RagA. Molecular masses are given in kilodaltons. (B) Representative immunofluorescence images showing HA-RagA localization to LAMP1-labeled lysosomes. Bars, 10 µm. Insets are 10.2 µm wide. (C) Representative immunofluorescence experiment showing the effect of starvation on FLCN lysosome localization in cells expressing HA-RagA. Bars, 10 µm. Insets are 10.2 µm wide. (D) Quantification of FLCN lysosome localization (percentage of cells with five or more FLCN spots that are also RagA positive; mean ± SEM; n = 3 experiments; 33–43 cells per condition; ***, P < 0.001; Student's t test).

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal