Loss of leupaxin promotes podosome turnover, and paxillin phosphorylation accelerates podosome turnover. (A) Lifetimes of single podosomes present at time point 0 were analyzed from time-lapse movies by measuring the time until they disappeared. The cumulative distribution of these measurements is shown. 10–30 podosomes were measured per cell. (B) Podosome lifetime was assessed as described in A and plotted as a cumulative distribution. 10–30 podosomes were measured per cell. This figure was reproduced from Fig. 4, D and E, in Klapproth et al. (2).