SORL1 transcript levels in vis but not sc human WAT correlate with BMI. (A–D) Levels of SORL1 transcripts were determined by quantitative RT-PCR in human vis and scWAT samples and calculated as relative to levels of 18S rRNA in the samples. Correlation of SORL1 transcript levels with BMI is seen in visWAT of males (A, n = 133) and females (B, n = 267), but not in scWAT of both sexes (C, n = 133; and D, n = 266). Significance of data was determined using linear regression analysis with Pearson correlation coefficient. (E) Levels of SORL1 transcripts in mature adipocytes were determined by quantitative RT-PCR in human vis and scWAT samples and calculated relative to levels of 18S rRNA in the respective sample. Data are given as mean ± SEM (n = 28 for scWAT, n = 34 for visWAT). Statistical significance of data were determined using two-sided Student’s t test; *, P < 0.05. (F)SORL1 transcript abundancy (transcripts per million [TPM]) in SVF cell types of human WAT. (G) Percent distribution of cell types in the SVF of human scWAT and visWAT. (H) Percent distribution of cell types in the SVF of human scWAT and visWAT among SORL1-expressing cells. Data in F–H are based on deposited single-cell RNA sequencing data (Vijay et al., 2020).