Mitochondrial stress increases biosynthesis of amino acids. (A) Venn diagrams showing commonly up-regulated transcripts (top) and proteins (bottom) with an FDR < 0.05. (B) Enrichment analysis of 59 transcripts (top) and 17 proteins (bottom) up-regulated were performed using KEGG pathways and are represented as negative of log10 of p-value after Bonferroni correction. Common enriched pathways in RNA sequencing and proteomics are represented in bold. (C) Graphical representation of enzymes and metabolites of de novo serine biosynthesis and their integration in cellular metabolism. Enzymes highlighted in red are up-regulated in RNA sequencing and in red bold in both RNA sequencing and proteomics. (D) Correlation of cytosolic and mitochondrial amino acid-tRNA synthetase (aaRS) genes and proteins in each stress condition. Values are represented as log2-fold changes (FC) relative to control. Dashes lines depict the 0 values to indicate the up- or down-regulation in each analysis. (E) Enrichment score plots from GSEA using a combine mitochondrial stress expression extracted from the RNA seq. 3PG, 3-phosphoglycerate; Acti, actinonin; Dox, doxycycline; FDR, false discovery rate; Glc, glucose; MB, MitoBloCK-6; NES, normalized enrichment score; PEP, phosphoenolpyruvate; PHGDH, phosphoglycerate dehydrogenase; PSAT1, phosphoserine aminotransferase 1; PSPH, phosphoserine phosphatase; Pyr, pyruvate; SDSL, serine dehydratase; Ser, serine; TCA, tricarboxylic acid.