Mitochondrial stress inhibits mitochondrial translation and decreases mitochondrial ribosomal proteins and OXPHOS complexes. (A) Venn diagrams showing commonly down-regulated genes (top) and proteins (bottom) with an FDR < 0.05. (B) Enrichment analysis of 101 proteins down-regulated was performed using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and represented as negative of log10 of p-value after Bonferroni correction. (C) A STRING network showing the enrichment in mitochondrial ribosomal proteins (MRPs; yellow) and OXPHOS proteins (blue). Line thickness indicates the strength of the association. (D and E) Correlation of transcript and protein data for the (D) MRPs and (E) OXPHOS components with each stressor. Values are represented as log2-fold changes (FC) relative to control. Dashes lines depict the 0 values to indicate the up- or down-regulation in each analysis. (F) Western blot analysis of OPA1 upon treatment for 24 h with each stressor. Long OPA1 isoforms (L-OPA1) and short OPA1 isoforms (S-OPA1) are indicated. Acti, actinonin; Dox, doxycycline; MB, MitoBloCK-6; NAFLD, non-alcoholic fatty liver disease.