Ssa1, Ydj1, or Hsp42 does not colocalize with the mNG-Pex15Δ30 foci formed by overexpression of Msp1 in doa10Δget1Δget2Δ cells. doa10Δget1Δget2Δ cells expressing Ssa1-mCherry (upper panel), mCherry-Ydj1 (middle panel), and Hsp42-mCherry (lower panel) from their own promoters and 3xFLAG-mNG-Pex15Δ30 from the GAL1 promoter with overexpression of Msp1 (Msp1O/E) from the ADH1 promoter were grown in SCD at 30°C and in SCGal for 4 h at 30°C and imaged by fluorescence microscopy. Single-plane images were shown. Scale bar, 5 μm. DIC, differential interference contrast microscopy.