mNG-Pex15Δ30 localizes to peroxisomes in GET-deficient cells. (A–C) WT (A), get3Δ (B), and get1Δget2Δ (C) cells expressing 3xFLAG-mNG-Pex15Δ30 from the GAL1 promoter were grown in SCD at 30°C and in SCGal for 4 h at 30°C and imaged by fluorescence microscopy. Peroxisomes were labeled with mCherry-PTS1. Single-plane images (upper panel) and maximum-projection images (lower panel) reconstituted from the z-stacks (25 plane stacks, 0.2-μm spacing) are shown. Scale bar, 5 μm. DIC, differential interference contrast microscopy.