Mim1 forms a channel. (A) Current recordings in symmetrical buffer conditions from a bilayer containing active Mim1 channels at the indicated voltage (V_m). (B) Current-voltage recordings of the Mim1 channel in symmetrical buffer conditions. (C) Mean variance plot of a single Mim1 channel unit calculated from the symmetrical current recording (A) at V_m = 120 mV. (D) Current-voltage recordings of the Mim1 channel without (black) or after coreconstitution with Mim2 (red). Mean values of V_rev of five (Mim1) and four (Mim1 + Mim2) independent experiments with SD (unpaired t test). (E) Mean of the maximal current of the Mim1 channel in the absence (n = 15) and presence (n = 11) of Mim2.