Figure 8.
Figure 8. HSP90 is required for PRPF8 stability in the cytoplasm. (A) Effect of GA on the levels of U5 proteins. Left images represent WBs made with extracts of HeLa cells expressing WT PRPF8-LAP and treated (+) or untreated (−) with GA. WBs were probed with the antibodies indicated at the right. On the right is a quantification of the signals as measured with ImageJ and expressed as percentages of the untreated controls. Values are means of three experiments. (B) Effect of GA on the levels of RPAP3-bound cytoplasmic PRPF8. Legend as in Fig. 5, except cells were treated with GA. Insets are magnified images of the dots marked by the white arrows. Bars: (main images) 10 µm; (insets) 1 µm. (C) Quantification of the green (PRPF8) and red (p54-RPAP3) signals in 30 dots shown at B. Error bars indicate SEM.

HSP90 is required for PRPF8 stability in the cytoplasm. (A) Effect of GA on the levels of U5 proteins. Left images represent WBs made with extracts of HeLa cells expressing WT PRPF8-LAP and treated (+) or untreated (−) with GA. WBs were probed with the antibodies indicated at the right. On the right is a quantification of the signals as measured with ImageJ and expressed as percentages of the untreated controls. Values are means of three experiments. (B) Effect of GA on the levels of RPAP3-bound cytoplasmic PRPF8. Legend as in Fig. 5, except cells were treated with GA. Insets are magnified images of the dots marked by the white arrows. Bars: (main images) 10 µm; (insets) 1 µm. (C) Quantification of the green (PRPF8) and red (p54-RPAP3) signals in 30 dots shown at B. Error bars indicate SEM.

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