AdoMet’s effect on SG is not linked to protein methylation. (A) Representative immunofluorescence images of HeLa cells stained for DDX3 and G3BP1 after 500 µM NaAsO₂ treatment. Before arsenite stress, HeLa cells were treated with DMSO or 20 µM AdOx for 48 h followed by the addition of 4 mM AdoMet for 3 h. Scale bar is 10 µM. (B) Box plot displaying the number of SGs/cell from A. (C) Box plot displaying quantification of the average SG size from A. (D) Western blot analysis from HeLa cell lysates that were treated with DMSO or 20 µM AdOx followed by addition of 4 mM AdoMet. Actin serves as a loading control. (E) Quantification of total ADMA levels from D. Data are presented as average ± SEM of three independent replicates. Box plots in B and C represent a compilation of three independent experiments. Statistical significance in B and C was determined by one-tailed unpaired Student’s t test (*, P < 0.05). For box plots, the ends of the box mark the 25th and 75th percentiles. The median is marked by a horizontal line inside the box. The whiskers mark the minimum and maximum measurements.