SGs are unable to properly fuse in AdoMet-treated HeLa cells. (A) Box plots depicting the number of SGs/cell in HeLa cells after the addition of 500 µM NaAsO₂. Gray boxes represent HeLa cells treated with AdoMet before stress. White boxes represent the untreated control. (B) Box plots depicting the average SG size at the indicated time points after addition of 500 µM NaAsO₂ for both AdoMet-treated (+AdoMet) and untreated (−AdoMet) HeLa cells. (C) Western blot analysis of HeLa cell lysates from the indicated time points after the addition of 500 µM NaAsO₂ from AdoMet-treated and untreated cells. Actin serves as a loading control. (D) Quantification of proteins levels from C. Data are presented as average ± SEM of three independent replicates. (E) Representative immunofluorescence images of HeLa cells stained for Tubulin and G3BP1 after treatment with 500 µM NaAsO₂-treated conditions. HeLa cells were treated with or without 4 mM AdoMet for 3 h and then DMSO or 5 µM nocodazole for 2 h before addition of NaAsO₂. Scale bar is 10 µM. (F) Box plot displaying the number of SGs/cell from E. (G) Box plot displaying quantification of the average SG size from E. Box plots in A, B, F, and G represent a compilation of three independent experiments. Statistical significance in A, B, F, and G was determined by one-tailed unpaired Student’s t test (*, P < 0.05). For box plots, the ends of the box mark the 25th and 75th percentiles. The median is marked by a horizontal line inside the box. The whiskers mark the minimum and maximum measurements.