Time-dependent effects of AdoMet treatment on arsenite-induced SG formation in mammalian cells. (A) Box plots displaying quantification of number of SGs/cell and average SG size of HeLa cells treated with or without 4 mM AdoMet at the indicated time point before 500 µM NaAsO₂ for 1 h. Box plots represent a compilation of three independent experiments. (B) Quantification of U2OS cells containing SGs treated with or without 4 mM AdoMet at the indicated time point before 500 µM NaAsO₂ for 1 h. Data are presented as average ± SEM of three independent replicates. In A, statistical significance was determined by one-tailed unpaired Student’s t test, and in B, one-tailed paired Student’s t test (*, P < 0.05). For box plots, the ends of the box mark the 25th and 75th percentiles. The median is marked by a horizontal line inside the box. The whiskers mark the minimum and maximum measurements.