AdoMet supplementation regulates SG assembly in HeLa and U2OS cells. (A) Representative immunofluorescence images of AdoMet-treated or nontreated HeLa cells stressed with 500 µM NaAsO₂, 100 µM MG132, or 2 µM RocA and stained for DDX3 and G3BP1. (B) Box plot displaying the number of SGs/cell and average SG size from A. (C) Western blot analysis from HeLa cell lysates of AdoMet-treated and untreated cells. Values represent normalized protein levels of AdoMet-treated to untreated samples. Actin serves as a loading control. (D) Representative immunofluorescence images of AdoMet-treated or untreated U2OS cells stressed with 500 µM NaAsO₂ or 2 µM RocA and stained for DDX3 and G3BP1. (E) Box plot displaying the number of SGs/cell and average SG size in response to arsenite stress from D. (F) Quantification of U2OS cells containing SGs in response to RocA treatment from D. Data are presented as average ± SEM of three independent replicates. Box plots in B and E are a compilation of three independent experiments. Scale bars in A and D are 10 µm. Statistical significance in B, E, and F was determined by one-tailed unpaired Student’s t test, and in F, one-tailed paired Student’s t test (*, P < 0.05). For box plots, the ends of the box mark the 25th and 75th percentiles. The median is marked by a horizontal line inside the box. The whiskers mark the minimum and maximum measurements.