Stress-specific and time-dependent effects of AdoMet treatment on acute-induced SG formation in yeast. (A) Quantification of azide-induced SG formation in non–AdoMet-treated cells (Control) or cells treated with 250 µM AdoMet at the indicated time point before azide addition. (B) Quantification and representative fluorescent images of logarithmically growing with (AdoMet) or without (Control) 250 µM AdoMet strains expressing Ded1-GFP upon glucose deprivation. (C) Quantification and representative fluorescent images of logarithmically growing with (AdoMet) or without (Control) 250 µM AdoMet strains expressing Ded1-GFP upon heat shock. (D) Polysome traces from logarithmically growing strains with or without 250 µM AdoMet under unstressed or glucose deprived (Glucose–) conditions. Bar graphs in A–C presented as average ± SEM of three independent replicates. Scale bars in A and B are 5 µM. Statistical significance in A and B was determined by one-tailed paired Student’s t test (*, P < 0.05).