Exogenous AdoMet suppresses acute stress-induced SGs. (A) Representative fluorescence images of endogenously expressing GFP-tagged SG proteins upon azide treatment after growing logarithmically in the presence or absence of 250 µM AdoMet. (B) Quantification of cells with foci from A. (C) Quantification of the number of foci/cells from A. (D) Western blot analysis from strains used in A. (E) Representative fluorescence images of endogenously expressed Ded1-GFP in WT and hmt1Δ strains upon azide treatment. Pgk1 serves as a loading control. (F) Quantification of cells with foci from E. (G) Quantification of the number of foci/cell from E. (H) Polysome traces from cultures that were subjected to azide treatment after growing logarithmically in the presence or absence of 250 µM AdoMet. Bar graphs in B, C, F, and G are presented as average ± SEM of three independent replicates. Scale bars in A and E are 5 µm. Statistical significance in B, C, F, and G was determined by one-tailed paired Student’s t test (*, P < 0.05).