Characterization of the SG phenotype in ado1Δ strains. (A) Quantification of cells with Ded1-GFP foci in WT, hmt1Δ, ado1Δ, and ado1Δ;hmt1Δ background strains at 1-d time point. (B) Quantification of cells with foci in WT and ado1Δ backgrounds expressing either WT or RK 4 Ded1-GFP. (C) Quantification of cells with foci for P-body markers (Dcp2 and Edc3) in WT and ado1Δ background strains at 1-d time point. (D) Representative images from D. (E) Quantification and representative fluorescent images of WT and ado1Δ strains with GFP-tagged metabolic enzymes at 1-d time point. *, P < 0.05. (F) Western blot analysis of strains used in E at 1-d time point. (G) Quantification of proteins levels from F. Pgk1 serves as a loading control. (H) Representative fluorescent images of WT or ado1Δ strains expressing Cdc19-GFP and Pbp1-mRuby at 1-d time point. (I) Quantification of the degree of colocalization of Cdc19-GFP foci to Pbp1-mRuby foci in WT and ado1Δ strains. (J) Representative fluorescent images of WT or ado1Δ strains expressing Cys4-GFP and Pbp1-mRuby at 1-d time point. (K) Quantification of the degree of colocalization of Cys4-GFP foci to Pbp1-mRuby foci in WT and ado1Δ strains. Bar graphs in A–C, E, G, I, and K are presented as average ± SEM of three independent replicates. Scale bars in D, E, H, and J are 5 µM. Statistical significance in E was determined by one-tailed paired Student’s t test (*, P < 0.05).