5′ UTR mRNA-associated SG proteins are not recruited to SGs under high AdoMet levels under growth to 1 d. (A) Quantification and representative fluorescence images of endogenously expressed GFP-tagged SG proteins in WT and ado1Δ strains at 1-d time point. (B) Western blot analysis of strains used in A at 1-d time point. Numbers below each mutant indicate clone number for that genotype. Pgk1 was used as a loading control. (C) Quantification of proteins levels from B. Protein expression for each protein is normalized to the WT sample. (D) Quantification of mRNA levels in WT and ado1Δ strains using qPCR analysis. (E) Quantification and representative fluorescence images of endogenously expressed Ded1-GFP in logarithmically growing WT and ado1Δ strains subjected to acute stress. (F) Quantification and representative fluorescence images of endogenously expressed Ded1-GFP in logarithmically growing WT and ado1Δ strains subjected to heat shock. Bar graphs in A and C–F are presented as average ± SEM of three independent replicates. Scale bars in A, E, and F are 5 µm. Statistical significance in A, C, and D was determined by one-tailed paired Student’s t test (*, P < 0.05).