Genetic disruptions of SAM1 and ADO1 result in changes in methionine biosynthetic pathway intermediates. (A) Quantification of methionine and AdoMet from WT, 250 µM-treated WT, SAM1 D121N, 250 µM-treated SAM1 D121N, and ado1Δ strains at 1-d time point. Values are normalized to WT samples. (B) Quantification of methionine and AdoMet from WT, 250 µM-treated WT, and ado1Δ strains at log phase. Bar graphs are presented as average ± SEM of three independent replicates. Values are normalized to WT samples. Statistical significance was determined by one-tailed paired Student’s t test (*, P < 0.05).