Recruitment of Sam1 to SGs is stress specific. (A) Diagram of methionine biosynthesis/recycling pathway in S. cerevisae.(B) Percentage of cells with Sam1-GFP foci and Ded1-GFP foci at different growth stages. Data are presented as average ± SEM of three independent replicates. (C) Percentage of logarithmically growing cells that form Ded1-GFP or Sam1-GFP foci upon exposure to different acute stresses. (D) Representative fluorescence images from C. (E) Percentage of logarithmically growing cells that form Ded1-GFP or Sam1-GFP foci in response to overexpression of SG nucleator proteins (Ded1 or Pbp1) or Sam1. (F) Representative fluorescence images from E. (G) Percentage of logarithmically growing cells that form Ded1-GFP or Sam1-GFP foci after a 10-min heat shock at 46°C. (H) Representative fluorescence images from G. (I) Representative fluorescence images of Sam1 colocalization with SGs (Ded1) under heat shock conditions. Bar graphs in C, E, and G are presented as average ± SEM of three individual replicates. Scale bars in D, F, H, and I are 5 µm.