Schematic of the approach to assessing cancer characteristics. (A) Intestinal organoids are established from transgenic mice carrying inducible oncoproteins. Their morphological features vary depending on the oncogenic proteins. (B) Oncogenic phenotypes of the organoids can be analyzed by well-established in vitro assays, such as gene expression, proliferation, metabolic, and apoptosis assays. (C) Organoids carrying inducible proteins enable nonbiased pathway analysis in the context of (combinations of) pathway inhibitors.