MASTL supports expression of cell movement– and actin cytoskeleton–related genes. (A) Venn diagram of differentially expressed transcripts (FDR < 0.05) measured with Illumina HT-12 at 24 h and 48 h after MASTL silencing in MDA-MB-231 cells. (B) Migration- and invasion-associated transcripts affected at RNA level after MASTL silencing according to Ingenuity Pathway Analysis. (C) A density scatterplot of SILAC proteome data displaying log2 of median protein ratios (siMASTL compared with siControl MDA-MB-231 cells) and total protein intensity. Each dot represents a protein. The color scale of the data point density is indicated on the right. Highlighted black circles represent significantly regulated proteins according to the significance B test. (D) List of differentially regulated hits identified in both the transcriptome and the total proteome datasets (shared identification). Blue, down-regulated hits; red, up-regulated hits. See also Fig. S3 and Table S2 and Table S3.