Figure S3.

MASTL silencing confirmed with Western blot and RT-PCR (for experiments related to Fig. 4 ). (A) Western blot analysis of MASTL and GAPDH 24 h and 48 h after silencing of MASTL#6 in samples used for the transcriptional analysis, MDA-MB-231 cells. (B) RT-PCR of MASTL relative to GAPDH 24 h and 48 h after silencing of MASTL#6 in samples used for the transcriptional analysis (n = 3 independent experiments, mean ± SD, unpaired t test, MDA-MB-231 cells). (C) Western blot analysis of MASTL and GAPDH 48 h after silencing of MASTL#6 in MDA-MB-231–labeled cells used for the total proteome analysis (MDA-MB-231 cells). H, heavy isotopes (Arg10, Lys8); L, light isotopes (Arg0, Lys0). See also Table S1.

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