Myr-WASp overexpression did not rescue the cortical F-actin and cell shape defects in spectrin mutant PECs. The z-projections at lateral domain (A and C) and z-projections at AJs (E–F). (A–A”) A Triton X-100–permeabilized pupal eye disc containing GFP-positive MARCM clones with α-spec RNAi and Myr-WASp overexpression was stained for phalloidin. Note the decreased cortical F-actin level in the mutant clones. Quantification of phalloidin membrane intensity is shown in B. (B) Mean phalloidin membrane intensity analyzed in A–A”. Data are means ± SEM (n ≥ 20 cells, representative of five animals). ****, P < 0.0001. (C–C”) A Triton X-100–permeabilized pupal eye disc containing GFP-positive MARCM clones with cpa/α-spec double RNAi and Myr-WASp overexpression was stained for phalloidin. Note the decreased cortical F-actin level in the mutant clones. Quantification of phalloidin membrane intensity is shown in D. (D) Mean phalloidin membrane intensity analyzed in C–C”. Data are means ± SEM (n ≥ 20 cells, representative of five animals). ****, P < 0.0001. (E–F”) Pupal eye discs containing GFP-positive MARCM clones with α-spec RNAi and Myr-WASp overexpression (E–E”) or with Myr-WASp overexpression only (F–F”) were stained for DE-cad to reveal cell shape. Note the increased apical area of 1° PEC with α-spec RNAi and Myr-WASp overexpression (arrowhead in E’) compared with wild-type 1° PEC (arrow in E’) in the same mosaic ommatidium. Also note that Myr-WASp overexpression alone did not affect apical area of 1° PEC (compare arrowhead and arrow in F’). Scale bars, 5 µm.