Immature autophagic compartments are more readily detected in axons than in dendrites. (A and B) En face images (A) and associated kymographs (B) of mCherry-GFP-LC3 in dendrites of a hippocampal neuron. Dashed white regions denote location of photobleaching. Arrows in B indicate the time of photobleaching. Throughout the figure, AVs positive for mCherry and negative for GFP fluorescence are denoted with red arrowheads in the mCherry channel and open green arrowheads in the GFP channel. AVs copositive for both mCherry and GFP are denoted with yellow arrowheads in both channels. Horizontal bars, 5 µm. Vertical bar, 10 s. (C) Kymograph analysis of mCherry-GFP-LC3 motility in an axon of a hippocampal neuron. Horizontal bar, 5 µm. Vertical bar, 1 min.