Figure 2. Discrete and periodic Plk4 ring patterns at the super-resolution scale. (A and B) Comparison of the resolution of conventional confocal and STED images of centriolar Plk4 (from different samples) with or without deconvolution (A) and the oval profile of Plk4 (B). Scale bars, 0.5 µm. (C) An array plot of all STED profiles of Plk4 (n = 37 cells). (D) Histogram of angles between nearest-neighbor pairs of Plk4 foci in discrete ring patterns. The angle shown in the inset STED image (*) corresponds to the distance indicated in yellow (*) in the profile in B. (E) Representative STED images and quantification of CEP152 spatial patterns. Details as for B and D. n = 30 cells. Scale bar, 0.5 µm. (F) Representative STED image and quantification of CEP192 spatial patterns. n = 10 cells. Scale bar, 0.5 µm.
Figure 2.

Discrete and periodic Plk4 ring patterns at the super-resolution scale. (A and B) Comparison of the resolution of conventional confocal and STED images of centriolar Plk4 (from different samples) with or without deconvolution (A) and the oval profile of Plk4 (B). Scale bars, 0.5 µm. (C) An array plot of all STED profiles of Plk4 (n = 37 cells). (D) Histogram of angles between nearest-neighbor pairs of Plk4 foci in discrete ring patterns. The angle shown in the inset STED image (*) corresponds to the distance indicated in yellow (*) in the profile in B. (E) Representative STED images and quantification of CEP152 spatial patterns. Details as for B and D. n = 30 cells. Scale bar, 0.5 µm. (F) Representative STED image and quantification of CEP192 spatial patterns. n = 10 cells. Scale bar, 0.5 µm.

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