Characterization of Drosophila ATP13A2 (dATP13A2). (a) Amino acid sequence alignment of human ATP13A2 (H. sapiens), mouse ATP13A2 (M. musculus), and Drosophila CG32000 (D. melanogaster). Amino acids in identity or similarity among the three different origins are highlighted in red or yellow, respectively. CG32000 shares 33% identity and 48% similarity with mouse ATP13A2, as well as 36% identity and 52% similarity with human ATP13A2. (b–d) dATP13A2 knockdown results in increased tubulin acetylation. Relative RNA levels of three independent Drosophila RNAi lines (HMS02719, GD14627, and HMS05242) driven by elav-gal4 and a WT control harboring elav-gal4 (Elav/+) are quantified by real-time PCR (b). **, P < 0.01; ***, P < 0.001, n = 3. Brain lysates of flies with either elav-driven dATP13A2 RNAi (RNAi HMS05242) followed by expressing human ATP13A2 (RNAi HMS05242, hATP13A2) or elav-driven dATP13A2 RNAi (HMS05242 RNAi; Ctrl) alone were immunodetected for acetyl-α-tubulin (Ac-tub) and α-tubulin (α-tub; c). Quantitative analysis of Ac-tub is shown (d). *, P < 0.05; **, P < 0.01, n = 3.