Induced Cdc14 release from the nucleolus is sufficient to redistribute GTP-Cdc42 between the mother and bud. (A) Representative time-lapse images showing Cdc14-GFP and Gic2-PBD in a bub2Δ cell arrested at S phase with hydroxyurea and overexpressing CDC5 from a copper-inducible promotor. Numbers indicate time in minutes, with 0 indicating the time of copper addition. Scale bar, 5 µm. (B) Timing of Cdc14 release and Gic2-PBD biosensor redistribution in bub2Δ cells arrested at S phase with hydroxyurea and overexpressing CDC5 (n = 100 cells from three experiments). (C) Representative time-lapse images showing Gic2-PBD redistribution in S phase in bub2Δ mutant cells overexpressing Cdc5 from a copper-inducible promotor. Numbers indicate time in minutes, counted from the time of copper addition. Scale bar, 5 µm. (D) Timing of Gic2-PBD redistribution in bub2Δ and in bub2Δ cdc14-1 cells overexpressing Cdc5 from a copper-inducible promotor and arrested in S phase at 37°C (n = 100 cells from three experiments for each genotype). (E) Representative time-lapse images showing Gic2-PBD redistribution in S phase–arrested bub2Δ cdc14-1 cells overexpressing Cdc5. Numbers indicate time in minutes, counted from the time of copper addition. Scale bar, 5 µm. (F) Gic2-PBD intensity of bub2Δ and bub2Δ cdc14-1 cells overexpressing Cdc5 from a copper-inducible promotor and arrested in S phase at 37°C. The intensity was measured at 60 min after copper addition (n = 100 cells for each genotype from three experiment). Asterisks indicate statistically significant differences (****, P < 0.0001, Mann-Whitney t test).