Loss of leupaxin and/or paxillin affect matrix degradation and cell migration. (A) IF images of DAPI-stained (magenta) +/+, leupaxin^−/−, paxillin^−/−, and paxillin/leupaxin dKO RAW cells seeded on Oregon Green 488–labeled gelatin (green) for 24 h. Scale bar, 100 µm. (B) Relative degradation capacity (area of degraded collagen normalized by number of nuclei) quantified from images shown in A. (C) Migration of these and K3^−/− RAW cells in relation to +/+ cells assessed by Transwell assays.