Figure 2. UBC-13, UEV-1, and CHN-1 act in the same pathway with VPS-34 to regulate phagosome maturation. (A–P) DIC and fluorescent images of cell corpses in the indicated strains expressing CED-1::GFP (A–C), GFP::RAB-5 (D–F), GFP::RAB-7 (G–I), LAAT-1::GFP (J–L), and YFP::2xFYVE (M–P). The head region of embryos at the twofold stage is shown. Cell corpses labeled by fluorescent markers are indicated by white arrowheads and unlabeled corpses are indicated by yellow arrowheads. Bars, 5 µm. (Q) The percentage of cell corpses labeled by phagosomal markers shown in A–L was quantified. (R and S) The percentage of cell corpses labeled by YFP::2XFYVE was quantified in the indicated strains. In Q–S, at least 15 embryos were scored in each strain. Data are shown as mean ± SD. One-way ANOVA with Tukey’s posttest was performed to compare mutant datasets with WT (Q) or datasets linked by lines (R and S). **, P < 0.0001; N.S., no significance.
Figure 2.

UBC-13, UEV-1, and CHN-1 act in the same pathway with VPS-34 to regulate phagosome maturation. (A–P) DIC and fluorescent images of cell corpses in the indicated strains expressing CED-1::GFP (A–C), GFP::RAB-5 (D–F), GFP::RAB-7 (G–I), LAAT-1::GFP (J–L), and YFP::2xFYVE (M–P). The head region of embryos at the twofold stage is shown. Cell corpses labeled by fluorescent markers are indicated by white arrowheads and unlabeled corpses are indicated by yellow arrowheads. Bars, 5 µm. (Q) The percentage of cell corpses labeled by phagosomal markers shown in A–L was quantified. (R and S) The percentage of cell corpses labeled by YFP::2XFYVE was quantified in the indicated strains. In Q–S, at least 15 embryos were scored in each strain. Data are shown as mean ± SD. One-way ANOVA with Tukey’s posttest was performed to compare mutant datasets with WT (Q) or datasets linked by lines (R and S). **, P < 0.0001; N.S., no significance.

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