Ldo proteins are required for normal LD morphology and lipophagy. (A) Size distribution of LDs of early stationary phase cells with the indicated genotype. (B) Levels of SE (left) and TAG (right) in cells with the indicated genotypes grown in SC medium to exponential (EXP), early (day 1; D1), and late stationary (day 4; D4) phases. The graph corresponds with the mean of two biological and two technical repeats. (C) Distribution of BODIPY-stained LDs (green) in cells with the indicated genotypes expressing endogenous Vph1-tdTomato (red) as described previously (Wang et al., 2014a). (D) Quantification of LDs localized to the vacuole in cells grown as in C at the indicated days. (E) Quantification of LDs localized to the vacuole in ldo16Δldo45Δ cells expressing the indicated plasmid-borne Ldo proteins and analyzed as in D. (F) Quantification of Lo domains at day 4 in cells with the indicated genotype grown as in C and expressing Vph1-GFP. Error bars indicate SD. Bars, 5 µm.