Figure 4. Deregulation of Ldo45 levels promotes Dga1-dependent TAG accumulation. (A) Quantification of neutral lipids in cells with the indicated genotype. Cells in YPGal were diluted to OD600 0.1 and grown for 24 h in the presence of 1 µCi/ml[1–14C] acetate. Neutral lipids were extracted and separated by thin layer chromatography. Graphs show the mean of two independent experiments. (B) Quantification of neutral lipids in cells with the indicated genotype as in A. (C) Protein extracts from cells grown as in Fig. 3 D analyzed by Western blotting with anti-Dga1 antibodies. The asterisk indicates a nonspecific band. EXP, exponential; STAT, stationary. (D) LD consumption in cells with the indicated genotype grown in YPD media to late logarithmic phase. Cerulenin (5 µg/ml) was added at time 0 to stimulate LD consumption. The graph displays the mean of three independent experiments. Error bars indicate SD.
Figure 4.

Deregulation of Ldo45 levels promotes Dga1-dependent TAG accumulation. (A) Quantification of neutral lipids in cells with the indicated genotype. Cells in YPGal were diluted to OD600 0.1 and grown for 24 h in the presence of 1 µCi/ml[1–14C] acetate. Neutral lipids were extracted and separated by thin layer chromatography. Graphs show the mean of two independent experiments. (B) Quantification of neutral lipids in cells with the indicated genotype as in A. (C) Protein extracts from cells grown as in Fig. 3 D analyzed by Western blotting with anti-Dga1 antibodies. The asterisk indicates a nonspecific band. EXP, exponential; STAT, stationary. (D) LD consumption in cells with the indicated genotype grown in YPD media to late logarithmic phase. Cerulenin (5 µg/ml) was added at time 0 to stimulate LD consumption. The graph displays the mean of three independent experiments. Error bars indicate SD.

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