Metabolic regulation of Ldo45 is required for LD morphology and protein targeting. (A) Ldo45 levels drop in stationary phase. Protein extracts from WT cells growing in SC medium at the indicated density were analyzed by Western blotting with anti-HA antibodies. (B) Quantification of Ldo45 and Ldo16 levels in WT cells analyzed as in A. The graph is the mean of three independent experiments. (C) LD morphology in cells overexpressing the indicated Ldo protein from the GAL1 promoter. (D) Quantification of LD aggregates in cells with the indicated genotype grown as in C. Quantifications are from three independent experiments for exponential (EXP) and stationary (STAT) phases, respectively. Error bars indicate SD. (E) Thin-section electron micrographs of cells expressing Ldo45 from the strong GPD promoter grown in YPD. (F) Cell overexpressing the indicated Ldo protein and endogenous Erg6-Cherry (left) or Dga1-GFP (right) were grown as in C for 16 h. Red and blue arrowheads indicate existence or absence of colocalization, respectively. Bars: (main images) 2 µm; (E, inset) 200 nm. DIC, differential interference contrast.