Figure 2. Specific role of Ldo45 in LD recruitment of Pdr16. (A) Localization of Pdr16-GFP in cells with the indicated genotype. Cells were grown in YPD to early stationary phase. Red and blue arrowheads indicate existence or absence of colocalization, respectively. (B) Localization of Pdr16-GFP in cells with the indicated genotype. Cells were grown in SC media to exponential phase and analyzed as in A. Existence or absence of colocalization is indicated as in A. Bars, 2 µm. DIC, differential interference contrast. (C) Pdr16-GFP levels in cells with the indicated genotype. Cells were grown as in B, and protein extracts were analyzed with anti-GFP.
Figure 2.

Specific role of Ldo45 in LD recruitment of Pdr16. (A) Localization of Pdr16-GFP in cells with the indicated genotype. Cells were grown in YPD to early stationary phase. Red and blue arrowheads indicate existence or absence of colocalization, respectively. (B) Localization of Pdr16-GFP in cells with the indicated genotype. Cells were grown in SC media to exponential phase and analyzed as in A. Existence or absence of colocalization is indicated as in A. Bars, 2 µm. DIC, differential interference contrast. (C) Pdr16-GFP levels in cells with the indicated genotype. Cells were grown as in B, and protein extracts were analyzed with anti-GFP.

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