Two Ldo isoforms interact with the seipin complex. (A) Scheme of YMR147W and YMR148W loci. The boxes indicate gene products from transcription through YMR147W (left) or YMR148W (right) promoters and that give rise to Ldo45 and Ldo16 proteins, respectively. The amino acid number of each protein is in parenthesis. In the left box, the splicing event giving rise to Ldo45 is depicted. Among the Ldo45 peptides detected by mass is one encoded by the intragenic regions (in light gray), confirming this splicing event. In black is the predicted hydrophobic hairpin involved in membrane association of both Ldo proteins. (B) Protein extracts from cells with the indicated genotype were analyzed by Western blotting with polyclonal antibody-recognizing epitopes on the C termini common to Ldo45 and Ldo16. (C) Ldo45 and Ldo16 coprecipitate with TAP-tagged Ldb16 and Fld1. Detergent-solubilized extracts prepared from cells with the indicated genotype were immunoprecipitated (IP), and eluted proteins were analyzed by Western blotting. Please note that the TAP-tagged proteins are recognized by the anti-Ldb16 rabbit IgG. The asterisk indicates a nonspecific band.