Deletion of BUD6 suppresses hof1Δ defects. (A) Yeast strains (fivefold serial dilutions) grown at 25°C or 34°C on YEPD plates for 2 d. (B) Growth rates of the same strains measured by optical density (OD₆₀₀) at 34°C in liquid culture (YEPD) in a shaking microplate absorbance reader. Data were averaged from eight independent trials. Lighter shading represents SEM. (C) F-actin organization in the same strains imaged by SIM before and after treating cells with the Arp2/3 complex inhibitor CK666 to remove actin patches. (D) Number of actin cable segments per cell determined by SOAX analysis, averaged from 10 cells per strain. (E) Number of cable intersections determined by SOAX analysis for same cells as above. (F) CV analysis on cable fluorescence after treatment with CK666, analyzed for 20 mother cells per strain. (G) Tortuosity of GFP-Sec4 secretory vesicle paths (n = 50 vesicles per strain). Error bars represent SEM. Statistical significance in all panels calculated by one-way ANOVA (n.s., not significant; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001).