PRD phosphorylation regulates INCENP MT binding and the SAC. (A) LAP-tagged hINCENP constructs. (B) Fluorescence images of cells expressing the indicated hINCENP constructs in taxol after knockdown of endogenous INCENP. Taken from a live imaging experiment. Green, GFP-INCENP; purple, RFP-H2B. Bar, 10 µm. (C) Immunofluorescence of the indicated constructs expressed over endogenous INCENP in cells treated with taxol. Bar, 2 µm. (D) DoM for cells undergoing unperturbed mitosis (left) and representative fluorescence images from the live imaging experiment (right). Representative of n = 3 independent experiments. Green, GFP-INCENP; red, RFP-H2B. Bar, 10 µm. (E) DoM for cells in taxol. Data were collected from the same experiment as Fig. 4 C. Representative of n = 3 independent experiments. (F) DoM for cells in taxol (left) and representative fluorescence images from the live imaging experiment (right). Green, GFP-INCENP; purple, RFP-H2B. Data from a single experiment. Bar, 10 µm.