Glucose represses CSR2 expression. (A) Cells expressing endogenously tagged Csr2-3HA were grown as indicated, and total cell lysates were immunoblotted with the indicated antibodies. Lact, lactate. (B) Total RNA was prepared from cells that are WT for CSR2, and the CSR2 transcript was detected by quantitative RT-PCR. Data represent the mean of four independent biological replicates (each with two technical replicates) ± SEM. (C) β-Galactosidase activity of cells expressing the lacZ reporter gene driven by the CSR2 promoter, grown as indicated. Data were normalized to β-galactosidase activity in glucose-grown cells and are the mean of three independent biological replicates (each with three technical replicates) ± SEM. (D) β-Galactosidase activity (± SEM) of WT, snf1Δ, and reg1Δ cells expressing the pCSR2:lacZ fusion. (E) β-Galactosidase activity (± SEM) of WT, mig1Δ, mig2Δ, and mig1Δ mig2Δ cells expressing the pCSR2:lacZ fusion. (F) WT, snf1Δ, and reg1Δ cells expressing endogenously tagged Csr2-3HA grown as indicated, and total cell lysates were immunoblotted with anti-HA and anti-PGK antibodies. (G) WT and mig1Δ mig2Δ cells expressing endogenously tagged Csr2-3HA grown as indicated, and total cell lysates were immunoblotted with anti-HA and anti-PGK antibodies.
